Evaluation of the anti-candidal property of a wild macro-fungus, Trametes lactinea, on clinically isolated Candida species




Objective:  Anti-candidal property of extracts of wild macrofungus Trametes lactinea on clinically isolated Candida species was investigated.

Methods: The extracts were then screened for their phytoconstituent composition qualitatively and quantitatively. In addition, the extracts were assessed for their anti-candidal efficacy against sixty-seven (67) Candida species isolated from high vaginal swabs of female patients attending ten (10) selected hospitals.

Results: Methanol had the best yield (3.2mg/g), while the least yield was with n-hexane (0.7mg/g). Saponin, tannin, steroid, terpenoid and cardiac glycoside were found in all the extracts. The methanol extract had the highest amount of cardiac glycoside (14.31mg/g), saponin (17.94mg/g) and tannin (3.56mg/g). The acetone extract had the highest steroid content (3.73mg/g), while the highest terpernoid (2.06mg/g) content was found in the n-hexane extract. For the anti-candidal efficacy of the extracts, the zones of inhibition produced by the macrofungus extracts against the Candida species ranged from 2.33 to 17.00mm, 4.33 to 12.33mm and 3.30 to 17.33mm for methanol, acetone and n-hexane extracts respectively. The highest (17.33mm) and least (2.33mm) inhibitory zone was produced against Candida albicans (A1) from Mother and child Hospital, Akure by the acetone and n-hexane extract of T. lactinea respectively.

Conclusions: Results from this study reveals that bioactive compounds with effective anti-candidal effect is present in extracts obtained from T. lactinea. The bioactive compounds may serve as a good alternative to anti-candidal drugs that are fast becoming ineffective.  


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